Glycomics comparison was carried out by screening melanoma serum biomarkers between C57 mice with and without B16 implanted. O-glycans were released from 10 μL sera by β-elimination, purified by Graphitized Carbon Cartridge Solid Phase Extraction (GCC-SPE) and analyzed by MALDI-QIT-TOF-MS. MS raw data were acquired and exported by Launchpad software. MATLAB was then applied for further data analysis. 10 Glycans were considered to have stable changes after B16 implantation and 5 of them were under structural analysis via MS/MS.
BAI Chen1,4, WU Gang1 & ZHAO Liang2,3 1Department of Life Science, Fudan University, Shanghai 200433, China
Serum was obtained from 25 male renal cell carcinoma(RCC) patients and 21 healthy males.O-glycans were released by a-elimination reaction and purified by graphitized carbon cartridge solid phase extraction,then profiled by matrix-assisted laser desorption/ionisation-time of flight mass spectrometry.After noise removal and peak alignment,1372 peaks were extracted from 200000 data points.Feature peaks were analyzed by calculation of differential sensitivity and specificity.The combination of two feature peaks was chosen as a biomarker and could clearly differentiate RCC and normal samples in our study group.
