目的:探讨氧化低密度脂蛋白(ox-LDL)对人冠状动脉内皮细胞表达可诱导共刺激分子配体(ICOSL)的影响。方法:以人冠状动脉内皮细胞为研究对象,通过间接免疫荧光、逆转录聚合酶链反应(RT-PCR)和Western blot等方法,观察ICOSL在人冠状动脉内皮细胞的表达及ox-LDL的干预作用。结果:对照组和ox-LDL刺激组ICOSL mRNA的平均光密度OD值分别为0.071±0.035和0.186±0.044(n=6),ICOSL Western blot吸光度A值分别为10.88±1.53和16.03±4.08(n=6),ox-LDL(100mg/L)可增加ICOSL在人冠状动脉内皮细胞中mRNA和蛋白的表达,并具有统计学意义(P<0.05)。结论:ox-LDL可上调人冠状动脉内皮细胞表达ICOSL。
The correlation of single nucleotide polymorphism (SNP) rs10569304 on the second expressed region of hole gene and congenital heart disease (CHD) of human being, and the effect of hole gene on CHD were investigated. 179 patients with CHD as CHD group and 183 healthy people as control group were selected in the case-control study. DNA was abstracted from the peripheral blood by phenol-chloroform method. Primer was designed for the flanking sequence of SNP rs10569304 on the second expressed region of hole gene. The genotype was identified by PCR degenerative acrylamide electrophoresis with amplification products. Then the three amplification products received sequencing. By chi-square test, the genotype frequency and allele frequency in CHD group and control group were analyzed. There was insertion-deletion (GCC/-) of SNP rs10569304 which corresponded to alleles of A and B in Southern Chinese people. The genotype frequency and allele frequency in control group and CHD group were met the Hardy-Weinberg equilibrium. By chi-square test, in control group and CHD group, the genotype frequency of AA (insertion homozygous), AB (insertion-deletion heterozygous) and BB (deletion homozygous) was 21.31%, 54.09%, 24.59% and 16.75%, 46.36%, 36.87%, respectively. The distributional difference of genotype frequency had statistical significance (χ2=6.51, P<0.05); The allele frequency of A and B was 48.36% and 51.64% in control group, 39.94% and 60.06% in CHD group, respectively. The distribu- tional difference of allele frequency had statistical significance (χ2=5.20, P<0.05). Meanwhile, by contrast with the control group, the BB genotype frequency and B allele frequency in CHD group was higher, but the AA and AB frequency was lower. There was higher risk to suffer from CHD involving B allele. BB genotype had 1.907-fold increased risk of developing CHD according to AA genotype (P<0.05). It is concluded that there is insertion-deletion (GCC/-) of SNP rs10569304 in the Southern Chinese people, and the people whose hole gene involv
