与钙传感器类钙调磷酸酶B蛋白(calcineurin B-like protein,CBL)互作的蛋白CIPK(CBL-interacting protein kinase)在植物特定的生长发育和应答胁迫过程中起重要作用。对前期研究得到的玉米ZmCIPK31基因构建原核表达重组载体,进行原核表达分析,转化重组质粒的大肠杆菌BL21菌株能够诱导出期望的目的蛋白。蛋白可溶性分析表明,它是可溶性的蛋白,通过淀粉树脂柱对其进行纯化,为下一步激酶活性分析提供了有活性的目的蛋白。同时,克隆了ZmCIPK31基因起始密码子ATG上游包括2189bp的启动子区段,顺式作用元件预测分析表明此区段不仅具有TATA-box和CAAT-box等启动子共有序列,还具有光应答、胁迫应答、发育相关、激素相关及其他功能未知的调控结构域。聚乙二醇(PEG)胁迫下,ZmCIPK31基因的诱导表达进一步表明其能够应答胁迫,且在地上部和根中的表达模式不同。
Ca2+作为植物中普遍存在的第二信使,其信号传感器CBL(calcineurin B-like protein)及与CBL互作的蛋白CIPK(CBL-interacting protein kinase)所构成的CBL-CIPK信号转导途径在转导特异Ca2+信号过程中起重要的作用。由于植物同时受多种复杂环境因素影响,因此必须同时对各种复杂因素作出响应并转导并存的信号。CBL和CIPK在结构、表达以及功能上的特异性构成了CBL-CIPK途径能够转导特异Ca2+信号的分子基础。本文在介绍CBL、CIPK的基础上,着重对其组合成的CBL-CIPK途径转导特异Ca2+信号的分子机制进行综述。
Drought stress is one of the most important factors limiting maize production. Rab17 is an ABA-responsive gene and associated with drought tolerance. In order to identify haplotypic structure and mine allelic variants at rab17 locus, nucleotide diversity and linkage disequilibrium (LD) structure of rab17 were evaluated among a mini core set of Chinese diversified maize inbred lines. Totally, 19 SNP and 18 insertion/deletions (InDels) were identified, among which 81% were in non-coding regions and 19% in coding regions. The results showed that a high level of diversity appeared within 1 kb upstream of the rab17 locus, and declined quickly downstream of the gene region. Rapid decay of linkage disequilibrium of rab17 region with distance within 1 kb was detected. Functional markers which can be developed based on haplotype 14 are expected to have contribution to molecular breeding for drought tolerance.
