[Objective]The paper was to culture the plasmodia of Physarum pusillum for obtaining the optimal condition of sporophores and sclerotium,to reduce the agricultural diseases.[Method]Liquid culture combined with oat-agar culture was used to culture plasmodia of P.pusillum.Then plasmodium was induced to obtain Sporophores in lab by hunger and adjusting light（3 000,6 000,9 000,12 000 lx）and temperature（20,22,24,26 ℃）.[Result]Sporophores and sclerotium of P.pusillum were obtained from both oat-agar media and liquid media.The optimal conditions were 26 ℃,6 000 lx and 22 ℃,3 000 lx.[Conclusion]The result provided theoretical basis for reducing the loss of crops caused by myxomycete as much as possible in agricultural production.
