Salmonella is a common genus of seriously harmful food-borne zoonotic bacteria. Humans and animals may be infected with Salmonella through ingestion of Salmonella-contaminated eggs and poultry meat. Therefore,in order to reduce the incidence of Salmonella infections,it is crucial to explore the pathogenic mechanism of Salmonella. invA and invE are major virulence factor genes that encode invasion proteins of Salmonella. In order to explore the pathogenic mechanism of Salmonella,phylogenetic analysis of major virulence factor genes in 33 Salmonella strains isolated from chicken was analyzed. According to the results,ivnA gene was successfully amplified from 33 Salmonella strains; ivnE gene was successfully amplified from 32 Salmonella strains. ivnA nucleotide sequences shared 72. 9%- 97. 6% homology among 12 sequenced Salmonella strains and shared 78. 9%- 97. 2% homology with those in GenB ank; ivnE nucleotide sequences shared over95. 3% homology among 23 sequenced Salmonella strains and shared 89. 6%- 98. 6% homology with those in GenB ank,which exhibited no genetic relationship to other organisms. This study provided the basis for rapid molecular detection,epidemiological research and molecular pathogenesis analysis of Salmonella.
VI型分泌系统(type VI secretion system,T6SS)具有转运效应蛋白分子到多种类型靶细胞的能力,广泛存在于革兰氏阴性菌,其结构复杂。当前T6SS结构模型表明其装置至少包含两个复合物:细胞膜跨膜相关装置和动态的噬菌体样结构。本文主要围绕T6SS分泌装置的结构、结构元件相互作用功能以及影响装置表达和活性的条件和信号来进行简要综述。
