The relationship between different BMPRIB genotypes of Small Tail Han sheep and FSHR and LHR mRNA levels duringthe oestrum was studied using semiquantitative PCR. The results indicated that FSHR mRNA extracted from the rightovary of BB (1.14 ± 0.11) ewes showed higher levels compared with AA (0.44 ± 0.11) and AB (0.36 ± 0.08) ewes (P < 0.01), andLHR mRNA extracted from the right ovary of BB (0.42 ± 0.02) ewes showed significantly higher levels compared with AA(0.23 ± 0.02) and AB (0.25 ± 0.04) ewes (P < 0.01); however, the mRNA extracted from the left ovary showed no significantdifference in levels among the genotypes during the oestrum. It indicated that the fecundity induced by a mutation ofBMPRIB in Small Tail Han sheep may be related to the changes of the mRNA expression of LHR and FSHR in ovary.
利用RACE和重叠延伸相结合的方法,从经晚疫病菌接种诱导的马铃薯水平抗性材料叶片中克隆了一个POTHR-1基因(potato Phvtophthora infestans-induced hypersensitive response related protein gene)的全长cDNA。序列分析表明,该基因编码225个氨基酸,与烟草harpin诱导蛋白基因hinl有很高的同源性(编码区核苷酸和氨基酸序列分别为83％和81％)。Southern杂交结果显示在马铃薯基因组中有2～3个拷贝。对其诱导表达模式研究表明:晚疫病病原菌接种36h后,该基因表达迅速增加;机械伤害及茉莉酸(JA)处理能够诱导表达;渗透胁迫(NaCl浸泡)能够诱导其微弱表达;但水杨酸(SA)不能诱导表达。该基因可能和病原与寄主互作时寄主产生过敏反应及细胞生理性死亡有关。
