[Object]The study aimed to discuss the decolorization on indigo dyeing wastewater by laccase from Coriolus versicolor.[Method]Firstly,the effects of temperature,pH,indigo concentration,HBT concentration,laccase dosage on the decolorization of indigo dyeing wastewater by laccase /HBT,and then the synergism of laccase and acid cellulase was analyzed.[Result]Using ABTS as the substrate,the kinetic parameters,K m and V max,were 0.318 mmol/L and 0.035 5 mmol/(L·min) respectively.The decolorization rate of indigo reached 96.5% when the laccase acted on indigo for 40 min with HBT as an introducer at temperature 50 ℃,pH =4.5,indigo concentration 100 mg /L,HBT concentration 0.1% and laccase dosage 100 IU /L.Due to the synergism of laccase and acid cellulase during the bio-finishing of blue jeans,the backstaining degree of blue jeans reduced by 85% when the amount of laccase added was 15 000 IU /kg.Menawhile,the synergism of the laccase and acid cellulase decreased indigo concentration in wastewater by 83.8%.[Conclusion] The laccase from Coriolus versicolor had a good prospect in the bio-finishing of blue jeans and the decolorization of indigo dyeing wastewater.
Enli GaoWeijia ZhongXiaolan FuFushan ChenZhigang Ye
