Contamination of deoxynivalenol(DON) in grains is common worldwide and pigs are particularly susceptible to this mycotoxin. The distribution of DON in porcine tissues following intravenous administration was investigated in this study. Fifteen pigs were randomly divided into three groups. Animals in groups A and B were administrated with DON at the dose of 250 and 750 μg kg–1 body weight, respectively, while group C served as blank control. Plasma, bile and 27 tissues were collected at 30 min post-administration. DON concentrations in all samples were tested using high-performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS). To observe the distribution of DON in tissues, these samples were further subjected to the immunohistochemical analyses. Totally, the bile and 13 tissues were sampled for DON-based detection, including kidney, mesenteric lymph nodes, muscle, stomach, jejunum, colon, plasma, spleen, rectum, cecum, liver, ileum, and duodenum. No significant difference was observed for the concentrations of DON in duodenum, ileum and liver samples between groups A and B; while the DON concentrations in cecum and rectum of group B were significantly higher(P-value <0.05) than those in group A. In addition, the DON concentrations in stomach, jejunum, colon, mesenteric lymph nodes, muscle, kidney, spleen, bile, and plasma of group B were remarkably higher than those of group A(P-value<0.01). Levels of DON in other 14 tissues including medulla oblongata, midbrain, diencephalon, pons, tip and tongue body, tongue, soft palate, tonsils, pharyngeal mucosa, oral buccal mucosa, thymus, thyroid, esophagus and adrenal gland were all below the limit of detection. The results of immunohistochemistry showed that 11 tissue samples(medullaoblongata, tonsil, adrenal medulla, thyroid gland, thyroid, stomach, duodenum, jejunum, kidney, spleen, and mesenteric lymph nodes) were positive and DON was mainly distributed around blood vessels in these tissues. Therefore, we believed that concentrations of DON in tissues
DENG Xian-baiDIN Huan-zhongHUANG Xian-huiMA Yong-jiangFAN Xiao-longYAN Hai-kuoLU Pei-chengLI Wei-chengZENG Zhen-ling
针对猪可食性组织中脱氧雪腐镰刀菌烯醇(DON)的残留,建立了一种液相-电喷雾质谱检测法.样品用纯水提取,C18柱净化,体积分数为30%的甲醇洗脱后进行LC-MS/MS分析.色谱柱采用Phenomenex Luna 5μC18(2),流动相采用体积比为3∶7的甲醇-体积分数为0.1%甲酸水溶液.质谱采用电喷雾离子源,正离子多重反应监测模式进行定量和定性分析.方法的最低检测限为25μg.kg-1,最低定量限为50μg.kg-1;在50、100、500μg.kg-13个水平的回收率为80.2%～99.4%,相对标准偏差为2.47%～9.47%.
