Powdery mildew caused by Blumeria graminis f. sp. tritici is one of the major wheat diseases worldwide. The Chinese wheat landrace Pingyuan 50 has shown adult-plant resistance(APR)to powdery mildew in the field for over 60 years. To dissect the genetic basis of APR to powdery mildew in this cultivar, a mapping population of 137 double haploid(DH) lines derived from Pingyuan 50/Mingxian 169 was evaluated in replicated field trials for two years in Beijing(2009–2010 and 2010–2011) and one year in Anyang(2009–2010). A total of 540 polymorphic SSR markers were genotyped on the entire population for construction of a linkage map and QTL analysis. Three QTL were mapped on chromosomes 2BS(QPm.caas-2BS.2), 3BS(QPm.caas-3BS),and 5AL(QPm.caas-5AL) with the resistance alleles contributed by Pingyuan 50 explaining 5.3%,10.2%, and 9.1% of the phenotypic variances, respectively, and one QTL on chromosome 3BL(QPm.caas-3BL) derived from Mingxian 169 accounting for 18.1% of the phenotypic variance.QPm.caas-3BS, QPm.caas-3BL, and QPm.caas-5AL appear to be new powdery mildew APR loci.QPm.caas-2BS.2 and QPm.caas-5AL are possibly pleiotropic or closely linked resistance loci to stripe rust resistance QTL. Pingyuan 50 could be a potential genetic resource to facilitate breeding for improved APR to both powdery mildew and stripe rust.
Muhammad Azeem AsadBin BaiCaixia LanJun YanXianchun XiaYong ZhangZhonghu He
The Italian wheat cv.Strampelli displays high resistance to powdery mildew caused by Blumeria graminis f.sp.tritici.The objective of this study was to map quantitative trait loci(QTLs) for resistance to powdery mildew in a population of 249 F 2:3 lines from Strampelli/Huixianhong.Adult plant powdery mildew tests were conducted over 2 yr in Beijing and 1 yr in Anyang and simple sequence repeat(SSR) markers were used for genotyping.QTLs Qpm.caas-3BS,Qpm.caas-5BL.1,and Qpm.caas-7DS were consistent across environments whereas,Qpm.caas-2BS.1 found in two environments,explained 0.4-1.6,5.5-6.9,27.1-34.5,and 1.0-3.5% of the phenotypic variation respectively.Qpm.caas-7DS corresponded to the genomic location of Pm38/Lr34/Yr18.Qpm.caas-4BL was identified in Anyang 2010 and Beijing 2011,accounting for 1.9-3.5% of phenotypic variation.Qpm.caas-2BS.1 and Qpm.caas-5BL.1 contributed by Strampelli and Qpm.caas-3BS by Huixianhong,seem to be new QTL for powdery mildew resistance.Qpm.caas-4BL,Qpm.caas-5BL.3,and Qpm.caas-7DS contributed by Strampelli appeared to be in the same genomic regions as those mapped previously for stripe rust resistance in the same population,indicating that these loci conferred resistance to both stripe rust and powdery mildew.Strampelli could be a valuable genetic resource for improving durable resistance to both powdery mildew and stripe rust in wheat.
Asad Muhammad AzeemBAI BinLAN Cai-xiaYAN JunXIA Xian-chunZHANG YongHE Zhong-hu
Stripe rust is a serious foliar disease posing a grave threat to wheat production worldwide. The most economical and environmentally friendly way to control this disease is to breed and deploy resistant cultivars. Zhongmai 175 is an elite winter wheat cultivar conferring resistance to a broad spectrum of Puccinia striiformis f. sp. tritici(Pst) races. To identify the resistance gene in the cultivar, genetic analysis was conducted using the parents, F1, F2 and F2:3 populations derived from the cross of Lunxuan 987/Zhongmai 175. Segregations in the F2 and F2:3 populations indicated a single dominant gene conferring resistance to stripe rust in Zhongmai 175, temporarily designated Yr ZM175. Bulked segregant analysis(BSA) with wheat i Select 90 K SNP array determined a preliminary location of Yr ZM175. Subsequently, Yr ZM175 was mapped on chromosome 2AS using simple sequence repeats(SSR), expressed sequence tags(EST) and newly-developed kompetitive allele specific PCR(KASP) markers, being flanked by Xgwm636 and Xwmc382 at genetic distances of 4.9 and 8.1 c M, respectively. Comparison of reaction patterns of Yr ZM175 on 23 Pst races or isolates and pedigree analysis with other genes on chromosome 2AS suggested that it is likely to be a new gene for resistance to stripe rust. The resistance gene and linked molecular markers will be useful in wheat breeding targeting for the improvement of stripe rust resistance.
LU Jia-lingCHEN CanLIU PengHE Zhong-huXIA Xian-chun
