目的观察补肾活血颗粒辅助治疗对帕金森病患者脑内多巴胺(DA)神经递质及多巴胺转运蛋白(DAT)的影响。方法 94例帕金森病患者随机分成对照组和治疗组,每组47例,两组均以西药美多巴为基础治疗,对照组加服安慰剂,治疗组加用补肾活血颗粒,疗程均为3个月。治疗前后采用脑电超慢涨落图分析仪检测两组患者脑内DA的变化情况,采用正电子发射断层扫描技术及感兴趣区(region of interest,ROI)分析治疗组患者纹状体DAT的改变情况。结果 (1)两组治疗前DA检测值均低于正常值,差异均有统计学意义(P<0.01),两组比较差异无统计学意义(P>0.05);两组治疗后DA值明显升高,与本组治疗前比较差异有统计学意义(P<0.01);与正常值比较差异均无统计学意义(P>0.05),两组比较差异有统计学意义(P<0.05),治疗组优于对照组。(2)治疗后治疗组患者纹状体内DAT放射性浓聚较治疗前明显增加,ROI分析治疗前后纹状体/小脑总比值分别为(1.86±0.32)和(2.61±0.53),差异有统计学意义(P<0.05)。结论补肾活血颗粒能提高帕金森病患者脑内DA水平,增加纹状体DAT含量,从而起到有效治疗帕金森病的作用。
Objective:To observe the clinical curative effect of Chinese medicine Bushen Huoxue Granule(补肾活血颗粒,BHG) on Parkinson's disease(PD) patients with depressive state.Methods:Sixty-two PD patients with depressive state were randomly assigned to two groups by using a random number table,31 in each group.Madopar was given to all as the conventional treatment.The fluoxetine hydrochloride dispersible tablet was given to the patients in the control group and BHG was given to those in the treatment group.The therapeutic course for all was 12 weeks.Before and after treatment,Hamilton depression rating scale(HAMD) was applied to judge the curative effect,and the changes of cerebral neurotransmitters levels in the brain of patients were detected by encephalofluctuograph technique.Results:The scores of HAMD in the two groups were decreased markedly after 12-week treatment.It was lower in the treatment group than that in the control group with significant difference(P<0.01).The contents of norepinephrine(NE) and 5-serotonin(5-HT) in the PD patients were obviously lower than normal value.There was no significant difference between the two groups before treatment(P>0.05).The contents of NE and 5-HT were all increased in the two groups after treatment(P<0.05),with significant differences between the two groups(P<0.01).Conclusion:BHG could increase the contents of NE and 5-HT in PD patients' brain to improve the depressive state of PD patients.
Objective:To explore the effect of Bushen Huoxue Decoction(补肾活血饮,BHD) on the orphan receptor(Nurr1) and tyrosine hydroxylase(TH) in the brain of rats with Parkinson’s disease(PD).Methods:One hundred and twenty SD rats were divided into 100 in the model group and 20 in the normal control group,fifty-eight SD rats from the model group,established into PD model successfully by injuring their substantia nigra (SSN) with 6-hydroxydopamine,were divided equally into the model group and the test group,and they were treated with saline and BHD,respectively,for eight successive weeks.The change in the rats’ behavior before and after treatment was observed by counting the cycles of rotation induced by apomorphine injection;the pathology of neurons,level of Nurr1 mRNA expression,and amount of TH positive cells in SSN were observed after treatment.Results:The rats’ behavior was improved in the tested group significantly,the rotation cycle after treatment being 84.0±20.0 cycles/40 min,which was significantly lower than that in the model group(377.0±62.3 cycles/40 min,P<0.01).Besides,the Nurr1 mRNA expression and TH positive cell in the test group were 0.97±0.15 and 49.40±14.72,respectively,which were significantly higher than those in the model group,0.22±0.03 and 5.45±2.58,respectively(all P<0.01).Conclusion:BHD could treat PD by enhancing the Nurr1 mRNA expression,increasing the TH content in brain,and promoting the repairing of injured neuron in cerebral SSN.
