[Objective] This study was conducted to clarify the biological information of PHYB genes in upland cotton （Gossypium hirsutum）. [Method] Two PHYB genes were identified from the genome database of allotetraploid cotton （G. hirsutum L. acc. TM-1）, and were found to be distributed on subgenomes A10 and D10. And then bioinformatic analysis on these two genes were performed. [Result] The PHYB genes of upland cotton had the same motifs and domains with the PHYB genes in other plant species, and even the number and location of the motifs and domains of these PHYB genes were consistent. The PHYB amino acid sequence alignment and the phylogenetic tree constructed based on PHYB amino acid sequence of these plant species indicated that the two PHYB genes in upland cotton had higher homology and closer evolutionary relationships with cocoa （Theobroma cacao）, but lower similarity to PHYB genes in monocotyledonous plants, such as rice （Oryza saitva） and corn （Zea mays）. The comparison of PHYB gene structure also revealed that plant PHYB gene was more conserved during evolution. The autophosphorylation of dozens of phosphorylation sites in upland cotton PHYB gene may be essential for the functions of phytochromes and plays a significant role in regulating phytochrome-mediated signal transduction pathways. [Conclusion] The results of this paper will provide a theoretical basis for the cloning and functional research of PHYB genes.
[Objective] The aim of this study was to identify the roles of an aquaporin gene GhNIP5.1 in upland cotton （Gossypium hirsutum） by bioinformatics method, so as to provide theoretical basis for further research on aquaporins in upland cotton. [Method] In silico molecular cloning was adopted to obtain an ORF sequence of GhNIP5.1 gene, which was then analyzed by the methods of bioinformatics. The coding region of GhNIP5.1 gene was obtained by analyzing the cotton genome se-quence published in NCBI. [Result] This cDNA sequence had a complete open reading frame of 897 bp and encoded 298 amino acid residues, including the con-served domain NPA （Asn-Pro-Ala） of MIP superfamily. The similarities of GhNIP5.1 deduced amino acid sequences from upland cotton with grape and Arabidopsis, were up to 89.3% and 83.2%, respectively. GhNIP5.1 was most similar in homology and 3-D structure of proteins to AtNIP5.1 among the nine members of NIP family in Arabidopsis. The coding region length of GhNIP5.1 gene was 2 067 bp, and it con-tained three introns and four exons. Al the exon-intron junctions of the gene con-tained the consensus splicing site pair GT-AG. [Conclusion] GhNIP5.1 gene probably has similar physiological functions with Arabidopsis AtNIP5.1.
