Objective To investigate the effects of 3,4-dichloroaniline(3,4-DCA) on activities of testicle enzymes as biological markers in rats.Methods Fifty male rats were randomly divided into 5 groups(n=10).One group was left untreated and used as a solvent control(administered orally by corn oil),while the other 4 groups were treated with 3,4-DCA.Corn oil was used as a solvent,and 3,4-DCA was diluted into tested concentrations(39,81,170,and 357 mg/kg).All the groups orally administered 3.4-DCA or corn oil,once a day for 4 weeks.The testicle tissue was homogenized in a 0.1 mol/L potassium phosphate buffer(0.1 mol/L,pH 7.2).The crude homogenate was centrifuged at 6000 rpm for 5 min at 4℃.The supematant obtained was used as an enzyme extract for determination of the enzyme activities.Results Compared with the contrl,the activities of ALP,ACP,and SDH were increased significantly at a lower level of 3,4-DCA,and decreased at a higher level of 3, 4-DCA,whreas the activities of LDH,LDH-X,and G6PDH were inhibited significantly with the increased 3,4-DCA concentration.Organ coefficient"organ weight/total body weight×100"of testis,liver,and spleen increased significantly with the increased 3,4-DCA concentration.These results suggest that 3.4-DCA toxicity to the male reproductive system was associated with the activities of testicular enzymes which are the sensitive biochemical endpoints reflecting 3,4-DCA toxicity to the male reproductive system.Conclusion 3,4-DCA has toxicity to the reproductive system in male rats.
Bo ZHANG~2 AND SEN LIN Department ofBiology,College ofApplied Sciences and Humanities,Beijing Union University, Beijing 100083,China
