Objective: Recent reports suggest that Cepharanthine(CEP),a natural alkaloid extracted from the roots of Stephania Cepharanthine Hayata,can inhibit the proliferation of various cancer cells,but few studies focus on its radiosensitization in nasopharyngeal carcinoma(NPC)cells. The aim of this study was to explore the radiosensitization effect and the potential mechanisms of CEP on nasopharyngeal carcinoma cell lines CNE-1 and CNE-2. Methods: The NPC cell lines CNE-1 and CNE-2 were treated with CEP(IC50)for 48 h before irration(IR);clonogenic survival was then assessed. The apoptosis and cell cycle progression were using flow cytometry.The DNA damage repair and cycle-regulating proteins were evaluated by Western blot analysis. Results: CEP could inhibit cell growth in both cell lines. The combination of CEP and radiation promote cell cycle G2/M phase arrest and apoptosis in CNE-1 and CNE-2 cells.DNA damage repair analysis showed that CEP has an inhibitory effect on DNA repair of CNE-1 and CNE-2 cells after radiation.Conclusion: CEP enhances tumor radioresponse through multiple mechanisms that may involve the halted cell cycle progression at G2/M phase and the inhibition of DNA repair after exposure to radiation.
Mengya ZhangTianjun MaQingfeng SongHuixia LinWei XiaoMeiqi ZhaoXinQing LiangYing Cui
Objective:To investigate the prevalence of metabolically healthy obesity(MHO)and its relationship with incidence of metabolic syndrome(MetS),type 2diabetes mellitus(T2DM)and hypertension in individuals in the city of Yulin.Methods:We studied 1,666 participants,aged over18years at baseline,with free of components of the MetS except waist criteria.Participants were divided into three groups based on body mass index(Ibm):lean/normal weight(Ibm<23kg/m2),overweight(Ibm,23-24.9kg/m2),obesity(Ibm≥25 kg/m2).The cumulative incidence of MetS,T2DM and hypertension over 5.21years among groups was assessed.Results:The prevalence of MHO was 19.5%in the baseline population.During an average 5.21year follow-up,the cumulative incidence of MetS,T2DM and hypertension in1,666participants were 16.4%,19.1%and 3.9%,respectively.The obesity group had a significantly higher cumulative incidence of MetS(16.4%vs.3.2%,P<0.001),hypertension(19.1%vs.3.7%,P<0.001),and T2DM(3.9%vs.1.6%,P<0.001)compared to the lean/normal weight group.Each kg/m2 of Ibm carried increased risk for T2DM(19%),hypertension(11%)and MetS(13%).Conclusion:Metabolically healthy obesity individuals confer increased risk for hypertension,T2DM and the MetS than their non-obese counterparts.
Huixia LinQingfeng SongMengya ZhangWei XiaoMeiqi ZhaoYing Cui
