Djuna Barnes, an American writer, is most associated with the development of English modernist writing of the twentieth century. Barnes's reputation as a writer was made when her second novel Nightwood was published in 1936 with an enthusiastic introduction by T.S. Eliot. Grounded mainly on queer theory, this paper analyzes Nightwood with its focus on the breakdown of the only two heterosexual marriages of the novel and intends to demonstrate that one of many aspects of the novel reflect the very core of queer theory.