The effect of magnetic field on α-amylase was studied. Under the experimental conditions, α-amylase solution was treated by 0.15 T, 0.30 T and 0.45 T static magnetic fields for a known period of time, then the activity, kinetic parameters, and the secondary conformation were investigated. The results showed that there was a considerable effect of the magnetic exposure on the α-amylase. The activity was increased by 27%, 34.1%, 37.8% compared with the control. It was also found that both kinetic parameters Km and Vm could be decreased due to the increasing magnetic field, Km decreased from 2.20×10-2 to 0.87×10-2, whereas Vm decreased from 2.0×10-3 g/min to 1.1×10-3 g/min. At the same time, there were some irregular changes in α-amylase secondary conformation.
To improve separate effect of binary heterogeneous azeotrope in the magnetic field with different magnetic induction intensity, the influence of magnetic field on the rectification process of binary heterogeneous azeotrope was investigated with 1-butanol-water system. The results show that the composition of liquid-liquid phase equilibrium of 1-butanol-water system has definitely changed, the composition of 1-butanol in light phase （1-butanol layer） increases by 1.1700-1.6300 and the composition of water in heavy phase （water layer） increases by 1.2100-1.5800 under the influence of magnetic field. By separation of magnetization, the composition of 1-butanol increases by 0.800-1.200 and the recovery ratio of 1-butanol increases by 1.600-2.500. Magnetic field has positive effect, however, the magnetized effect is not in proportion to magnetic induction intensity and has an optimum condition, in the range of 0.25 T-0.3 T.
