Objective： To investigate the expressions of heat shock protein （hsp） 10, hsp27, hsp60, hsp70 and hsp90a in esophageal squarnous cell carcinoma （ESCC） and normal tissues along the incisal margin （TIM）, and discuss the clinicopathologic features about their expressions. Methods： 120 specimens from ESCC and 36 specimens from TIM were made into tissue chips. The presence and the levels of expression of hspl0, hsp27, hsp60, hsp70 and hsp90a were observed on tissue chips by immunohistochemistry EnVision^TM. Their correlations to clinicopathologic features were analyzed. Results： The positive staining rates of hsp10, hsp27, hsp60, hsp70 and hsp90a in ESCC and TIM were 53.8% and 37.5%, 62.0% and 42.1%, 92.7% and 63.2%, 57.9% and 22.2%, 33.7% and 18.5% respectively. There were no statistical significances between the differential expressions of hsp10, hsp27 and hsp90a in ESCC and TIM （P 〉 0.05）, but there were great statistical significances about hsp60 and hsp70 （P 〈 0.01）. The level of hsp27 declined with the lower grade of differentiation of ESCC （P 〈 0.05）. Except for hsp27, the positive expressions of the other four HSPs had no correlation to the clinicopathologic features of ESCC. Conclusion： The expressions of hsp10, hsp27, hsp60, hsp70 and hsp90a in ESCC and TIM were a common event. The levels of hsp60 and hsp70 in ESCC were higher than those in TIM. The level of hsp27 declined with the lower grade of differentiation of ESCC showed that it may be play a role in the differentiation of ESCC.
目的研究热休克蛋白10(heat shock protein 10,HSP10)在大肠癌发生、发展过程中的表达规律,了解其与大肠癌临床病理特征和术后生存期的关系。方法应用免疫组化EnVision二步法,检测HSP10在大肠正常黏膜、腺瘤和腺癌中的表达,及其与临床病理表现和术后生存期的相关性。结果HSP10在大肠正常黏膜、腺瘤和腺癌中均有表达,在腺瘤、腺癌中的表达明显强于正常黏膜(P<0.001);HSP10的阳性表达与大肠癌患者的年龄、性别、肿瘤浸润深度、局部淋巴结转移、远处转移、临床分期、组织学分化程度等临床病理特征和术后生存期均无关。结论HSP10在大肠腺瘤和腺癌的高表达,提示可能与大肠癌的发生发展有关;HSP10的阳性表达与大肠癌的临床病理特征和术后生存期无关。
