Two new oleanane-type triterpenoids, parvifolactone A(1) and rubuside P(2), together with 11 known triterpenoids, fupenzic acid(3), 18,19-seco,2α,3α-dihydroxyl-19-oxo-urs-11,13(18)-dien-28-oic acid(4), euscaphic acid(5), maslinic acid(6), 1β-hydroxyeuscaphic acid(7), 2α,3α,19α,23-tetrahydroxyolean-12-en-28-oic acid(8), 2α,3β,19α,23-tetrahydroxyurs-12-en-28-oic acid(9), glucosyl pinfaensate(10), rubuside J(11), 2α,3α,19α,23-tetrahydroxyurs-12-en-24,28-dioic acid(12), and 2α,3β,19α- trihydroxyurs-12-en-23,28-dioic acid(13), were isolated from the roots of Rubus parvifolius.
以黄酮类化合物生物合成过程中的两个关键酶:4-香豆酰辅酶A连接酶(4-coumarate coenzyme A ligase,4CL)和查尔酮合成酶(chalcone synthase,CHS)为工具酶,探讨酶催化组合合成在合成"非天然的天然产物"群(库)中的应用.结果发现在4CL和CHS的共同作用下,仅利用8个简单的有机酸就可以产生包括8个黄酮类似物、16个4-羟基-δ-内酯和1个苄基丙酮共计25个"非天然的天然产物"群,为构建结构多样的小分子化合物群(库)提供方法学参考.
