Human umbilical cord blood (CB) has recently been used as a source of stem cells in transplantation. NK cells derived from CB are the key effector cells involved in graft-versus-host disease (GVHD) and graft-versus-leukemia (GVL). It was reported that the activity of CB NK cells was lower than that of adult peripheral blood (PB) NK cells. In this study,we analyzed the expression of some NK cell receptors and cytotoxicity-related molecules in CB and PB NK cells. The expressions of activating NK receptors,CD16,NKG2D and NKp46,did not show significant difference between CB and PB NK cells. But the expression of inhibitory receptor NKG2A/CD94 was significantly higher on CB NK cells. As to the effector function molecules,granzyme B was expressed significantly lower in CB NK cells,but the expressions of intracellular perforin,IFN-γ,TNF-α and cell surface FasL and TRAIL did not show difference between CB and PB NK cells. The results indicated that the high expression of NKG2A/CD94 and low expression of granzyme B may be related with the reduced activity of CB NK cells.
Yanyan Wang1,2,Han Xu1,2,Xiaodong Zheng1,Haiming Wei1,Rui Sun1 and Zhigang Tian1,31Institute of Immunology,Hefei National Laboratory for Physical Materials at Microscale and School of Life Sciences,University of Science and Technology of China,Hefei 230027,China
