[Objective] The aim of this study was to reveal the mechanism of Cu2+ hyper-accumulator in Setcreasea purpurea Boom from the angle of distribution characteristics and binding form of Cu2+ in tissue cells.[Method]The distribution characteristics of Cu2+ in subcells of Setcreasea purpurea Boom was studied by the technique of differential centrifugation,and the binding form of Cu2+ in roots and leaves of Setcreasea purpurea Boom was also investigated by the sequential chemical extraction method and the enzymolysis method.[Result]Cu2+ in roots mainly distributed in the cell wall which is accounting for one third of the Total Cu2+ in roots,while Cu2+ in leaves mainly distributed in the chloroplast which is accounting for a quarter to the Total Cu2+ in leaves.Under the high concentration of Cu2+ or the extended treatment duration,the translocation of Cu2+ in root cells into the cell wall increased but the translocation of Cu2+ in root cells into the plastid decreased,while the translocation of Cu2+ in leaf cells into the chloroplast increased but the translocation of Cu2+ in leaf cells into the cell wall decreased.Cu2+ in leaves was mainly combined with amino acid,small molecular polymeric pigments,protein and polysaccharide,while Cu2+ in roots was mainly combined with cell wall substances such as cellulose and membrane-bound protein.[Conclusion]The distribution characteristics and binding form of Cu2+ in cells is possibly one of the dominant mechanisms for Cu2+ hyper-accumulator in Setcreasea purpurea Boom.
