目的:研究欣胃颗粒及其拆方对胃癌前病变大鼠Wnt-1及β-catenin的影响。方法:实验分成空白组、模型对照组、胃复春组,益气组、养阴组、化瘀组、解毒组、益气养阴组、益气化瘀组、益气解毒组、养阴化瘀组、养阴解毒组、化瘀解毒组、益气养阴化瘀组、益气养阴解毒组、益气化瘀解毒组、养阴化瘀解毒组、益气养阴化瘀解毒(即欣胃颗粒)组。采用以MNNG为主的四因素联合造模法建立胃癌前病变的大鼠模型,空白对照组、模型对照组给予等量生理盐水灌胃,而其它16组同时给予相应药物灌胃,在16周造模结束后处死大鼠并取材,观察各组大鼠胃粘膜病理改变;采用Real Time PCR技术,检测胃黏膜Wnt信号通路关键因子Wnt-1、β-catenin的基因表达情况。结果:与空白组比较,模型组Wnt-1及β-catenin的基因表达水平明显增高,其差异具有显著性(P<0.05);中药各组与模型组比较,Wnt-1及β-catenin的表达水平下调,具有统计学意义(P<0.05)。结论:四法组的干预作用优于三法、两法、及单法组,表明各法之间具有协同增效作用;欣胃颗粒可改善胃癌前病变大鼠胃黏膜病理状况,下调Wnt-1及β-catenin的基因表达水平。
Objective:To observe the clinical effect of electroacupuncture on postpartum urinary retention(PPUR)through a randomized controlled study.Methods:Seventy-two PPUR puerperae who met the inclusion criteria were divided into the observation group(electroacupuncture at Ciliao(次髎BL 32) and Zhongliao(中髎 BL 33) group) and the control group(sham acupuncture group) according to the random number table method,with 36 cases in each.Urine catheters were retained.After 3 days of treatment,the therapeutic effect of PPUR was compared between the two groups.Results:In the observation group,the first urination time was significantly earlier than that in the control group,the first urination volume was significantly more than that in the control group,the bladder residual urine volume was significantly less than that in the control group,and the urinary infection was significantly less than that in the control group,the differences were statistically significant(all P <0.001).Compared with the control group,there were statistically significant differences in catheter removal,independent urination,cured cases,effective cases,ineffective cases,and total effective cases in the observation group(all P <0.05).Conclusion:The therapeutic value of electroacupuncture at BL32 and BL33 for primiparae with PPUR is significantly obvious.This therapy is worthy of clinical promotion.
