本研究工作通过荧光光谱法研究了阿斯匹林与单、双链 DNA 的相互作用,同时,利用原子力显微镜(AFM)对 DNA 与阿斯匹林作用前后的纳米形貌,进行了表征。在此基础上,我们研究了纳米材料胶体金对阿斯匹林与 DNA 相互作用的影响。研究结果表明,阿斯匹林能够识别单、双链 DNA,且与双 DNA 有着较强的相互作用;胶体金的存在可显著增强阿斯匹林对 DNA 分子的识别作用。
Mutations in cardiac troponin I （cTnI） gene were assessed based on gene-chip technology.Special probes were designed to fabricate the low-density gene-chip,which could detect the mutations in exons 3,5,7,and 8 of the cTnI gene simultaneously.For each exon,two oligonucleotide sequences labeled with fluorescein at the 5＇-end were designed,one （oligonucleotide Ⅰ） simulating the wild type and the other （oligonucleotide Ⅱ） simulating the mutant.Oligonucleotides Ⅰ and Ⅱ were mixed together to simulate the heterozygote.After optimizing the hybridization protocols,the fabricated gene-chip could detect the mutations in the exons of the cTnI gene with relative high sensitivity and specificity.The fully complementary probe gave a fluorescent signal almost 50% stronger than that of the one-base mismatched one,which is in accordance with the result from a theoretical estimate. An applicable special gene-chip is available to investigate and diagnose familial hypertrophic cardiomyopathy （FHCM） after further improvement.
