Tumor-associated carbohydrate antigens （TACAs）, important molecular markers on tumor cells, are major targets for the development of anti-cancer vaccines. However, due to the immunotolerance and weak immunogenicity, native TACAs cannot induce powerful immune response. The use of modified TACAs such as N-glycosides could be an alternative strategy to overcome this problem. Herein, the synthesis of N（OMe）-linked analogue of tumor-associated disaccharide antigen Thomsen-Friedenreich （TF） by the glycosylation reaction is described.
