Studies on the thermokinetic properties of model compound of Purple acid phosphatases (Na2[Fe2(μ-O)(μ-OAC)2(IDA)2].5H2O hydrolysis of ATP, at 37℃, pH=6.0 by usingLKB-2107 Batch microcalorimentry system. The Michaelis constant Km=(2.898 ± 0.195)x 10-4mol.L-1 and rate constant k2=(4.617±0.193) x 10-3.s-1 have been determined. The result hasbeen discussed.
Cell death manner is related to the contents of intra cell energy substances. The rate constants and the half life time of ATP, phosphocreatine (PCr) and inorganic phosphorus (P i) in different cells of eel and crucian during their death have been determined with 31 P NMR. It has been found that there are differences in rate constants and change rule of these energy substances for different animals and different cells in the same animal from this study. The decreasing order of the half life time is: muscle of eel>brain of eel>muscle of crucian for ATP; brain of eel≈muscle of eel>muscle of crucian for PCr. These results are very vital to understanding the concerned change pattern of energy substances and the speed of cell death.
