A new method for determining vancomycin was established based on the principle of fluorescence quenching.The mechanism of the fluorescence quenching was discussed.A linear relationship is obtained between F0/F and the concentration of vancomycin in the range of 40.0—450.0 μg/mL with the correlation coefficiency 0.9991.The detection limit was 10.91 μg/mL and the relative standard derivative was 0.70%.The effects of pH,foreign ions,standing time on the determination of vancomycin were examined.The results obtained by this method were agreed with those by the official method.
