Several Mn(Ⅲ)10-(hydroxylphenyl)-5,15-bis(pentafluoro-phenyl)corroles with the hydroxyl at ortho-,meta-and para-position of 10-phenyl group were used as the catalysts in the oxidative cleavage of plasmid DNA in the presence of H2O2.The catalytic system was composed of 10 μL 0.5 mg/mL pC DNA 6 in TE buffer solution(pH=8.0,4.0×10-2 mol/L Tris,1.0×10-3 mol/L EDTA),10 μL 1.0×10-5 mol/L Mn(Ⅲ)corrole in DMSO and 10 μL 0.3% H2O2.After 8 h incubation at room temperature,significant oxidative DNA damage could be observed with the ortho-hydroxyl Mn(Ⅲ)corrole showing a better activity as detected by agarose-gel electrophoresis.Plasmid DNA was completely damaged after 12 h incubation.These observations show Mn(Ⅲ)corrole can be used as an artificial model of nuclease.
Homogeneous solution of poor soluble aza bridged bis phenanthroline macrocyclic compound(H 2HAPP) was obtained by adding K 2CO 3 to its trifluoroacetate in CH 3OH CH 2Cl 2[ V (CH 3OH)∶ V (CH 2Cl 2)= 1∶1] solution. Coordination reaction between H 2HAPP and metal ion Zn(Ⅱ) in CH 3OH CH 2Cl 2[ V (CH 3OH)∶ V (CH 2Cl 2)=1∶1] solvent was studied by UV Vis spectroscopic method, which indicated 1∶1 complexing between H 2HAPP and Zn(II) ion occurred easily in such a homogeneous system at room temperature. And the formation of ZnHAPP complex was further confirmed by FAB MS and 1H NMR spectra.
