MtDNA was successfully extracted from 31 ancient individual bones at the tombs of Turfan basin and Lubunouer. Through four overlapping primers, we got 30 nucleotide sequences of 363 bp length. Intermediate nucleotide diversity, and genetic distances, as well as mean pairwise variance, are compatible with ancient Turfan mtDNA pool being an admixture of eastern Asian and European lineages. Such phenomenon shows that prior to the time of ancient Turfan, an ancient mingling of Euro-Asian population in Turfan basin had existed. The ancient Luobunuoer was the relic of Xinjiang that was found in the earliest time and at the farthest orient. The mtDNA sequences of ancient Luobunuoer are consisted by European lineage completely, no trace of mixture with Asian lineage. From nucleotide diversity, and genetic distances and mean pairwise variance, it has close relationship with European populations.
Ancient DNA molecules were successfully extracted from remains of Laoshang t omb. PCR amplification and sequencing analysis of mitochondrial hypervariable re gion Ⅰ (16035-16398) were carried out to determine its haplogroup. The results indicate the sequence belongs to M haplogroup attributed to Asian which consist s with the study of physical anthropology. The project acertained its lineage an d meanwhile enriched the database of ancient populations in China.
Mitochondrial DNA has been successfully extracted from 10 specimens from tour tombs at Jiangjialiang. Hypervariable region I (HVRI) in the mitochondrial DNA has been amplified and sequenced. Altogether 10 distinct haplotypes with 15 polymorphic sites have been obtained. A phylogenitic tree of the 10 sequences has been constructed by means of the maximum likelihood method. Four clusters,corresponding to the four tombs, have been presented in the tree. The fact that mitochondrial DNA is inherited exclusively in the female line and the features of graves in matriarchal society suggest that the Jiangjialiang population should not be taken to be a matriarchal community.
