An isolate of PVS,HZ00P1,was obtained from nature infected Solanum tuberosum from Hangzhou,Zhejiang province.It was primarily identified by DAS-ELISA and host reaction tests.3’ end partial sequence of the virus isolate was cloned.Nucleic acid sequence of the cp gene and deduced cp amino acid sequence alignments were compared with 16 other isolates registered in GenBank.The results showed that the 17 PVS isolates were divided into two groups.Among them,two Andean isolates were classified to groupⅡ,HZ00P1 and the other 14 isolates were assigned to groupⅠ.Compared with other PVS isolates in group Ⅰ,PVS HZ00P1 had 93.1%-98.1% and 95.9%-99.3% homologies of nucleotide sequence and deduced amino acid sequence respectively,whereas its similarities to the groupⅡwere 81.4%-81.7% and 93.5%-93.9%.A survey of PVS occurrence in Zhejiang province was achieved by RNA spot hybridization(RSH).Among the 30 samples collected from different areas of the province,26.7% were found to have the infection of PVS.It is concluded that PVS has become a common virus in Zhejiang province.
为更直观把握食品科学领域的核心学术脉络,本文利用web of science核心合集数据库和InCites分析工具,对2007—2016年10年间全球食品科学领域发表的科研论文进行多角度分析,同时对发展趋势进行预测。结果表明,过去10年间美国发文总量和总被引频次位居全球第一,而自2013年开始中国年发文量已跃升全球第1位。通过模型预测得出到2026年中国的论文总数将达到美国的近3倍,成为论文发表第一大国。数据还表明,中国在食品科学与技术研究领域虽呈现良好的活跃度,相对于全球平均水平的影响力、学科规范化的引文影响力(CNCI)也均高于全球平均水平,是全球食品科学技术领域的研究大国,然而综合TOP10%论文数、高被引论文等各项影响力指标,总体上仍低于美国与西班牙等国家,说明距离该领域的研究强国还有一定差距。今后应注重于原创和论文的质量。
