To reveal variation of organic matter content in red paddy soil, Through a 27 years-located fertilization experiment in red paddy soil, the content and composi- tion of organic matter in paddy soil were studied in this paper. The result showed that： the dynamics of soil organic matter of the different fertilization treatments showed significant differences, in the premise of equal nutrient （nitrogen and phos- phorus and potassium）, combining application of organic-inorganic was benefited for the accumulation of organic matter in paddy soil than without fertilizer treatment or chemical fertilizer treatment; the dynamics of soil humic acid, HA and FA of chemi- cal fertilizer only and Combining application of organic-inorganic treatments had basi- cally the same trend, But the contents of humic acid, HA and FA of combining ap- plication of organic-inorganic treatments had been higher than that without fertilizer and chemical fertilizer treatment. Moreover combining application of organic-inorganic treatments was benefited for improving the contents of HA and FA, but decreasing HA/FA ratio as an extension of time. Combining application of organic-inorganic was benefited for improving the contents ol readily oxidizable organic matter. And the contents of soil organic matter in long-term experiment and the contents of readily oxidizable O.M were highly significant positive correlation and the contents of soil total nitrogen, avail nitrogen and potassium were significant positive correlation; the contents of soil readily oxidizable O.M and the contents of soil total nitrogen, avail- able P and rice yield were significant positive correlation. Thereinto, the correlation （r=0.818 1 ） between the rice yield and soil readily oxidizable O.M was higher than the correlation （r=0.802 0） between the rice yield and soil organic matter. It showed the soil readily oxidized organic matter had greater contribution to the rice yield.
