Time-of-flight mass spectrometry (TOF-MS) is widely used in protein chemistry and proteomics research due to its high sensitivity , resolution and accuracy. Recently we have developed two Q-TOF-MS analytical systems titled as “ESI-TOF-MS detector of HPLC”, which have promising features and arrive at the coordinative level compared with the congener overseas production. The ESI-Orthogonal Acceleration-Reflecting-TOF mass spectrometry can detect the lysozyme sample concentration below 1 pmol/μL under the optimized conditions. The mass range of these instruments is from 50 to 6000dahon with a general ESI interface to the HPLC. The optimal mass resolution exceeds 6000(FWHM)and the signal/noise ratio of Reserpine (10 ng/L) is greater than 100.
