[ Objective] The research aimed to study the effects of Chanfukang on the cytokines of immunosuppressive mice, providing the basis for the further research of its effects on immune function. [ Method] Immunosuppressive mouse model was established through twice intraperitoneal in- jection with cyclophosphamide at the dose of 40 mg/kg. After the mice were gavaged respectively with Chanfukang at the dose of 10, 15 and 20 g/kg, the level of cytokines （IL-1β, IL-2 and TNF α） were detected. [Result] Chanfukang significantly increased the level of IL-1β, IL-2 and TNF in the serum of mica gavaged only with Chanfukang; the level of IL-1β, IL-2 and TNF α was inhibited in mice treated with cyclophosphamide; Chanfukang significantly increased the level of IL-1β, IL-2 and TNF α in the serum of immunosuppressive mice. [Condudon] Chanfukang should antagonize the immunosuppression of cyclophosphamide and improve the immune function.words
目的:研发奶牛抗菌肽生物制剂,防治奶牛子宫内膜疾病。方法:采用酸性尿素聚丙烯酰胺凝胶电泳和琼脂糖弥散法检测子宫内膜组织酸溶性提取物抗菌活性;应用琼脂糖电泳及水平电泳洗脱分离出相应的抗菌条带,HPLC进一步分离纯化,琼脂糖弥散法检测纯化物的抗菌活性,Tricine-SDS-PAGE电泳检测其分子量。结果:粗提物中有两种主要的抗菌成分,其对金黄色葡萄球菌26003、大肠杆菌BL-21、牛致病性大肠杆菌4605、绿脓杆菌10104具有抗菌活性。上层条带的主要色谱峰出现在43、45 min,其中45 min收集的样品有较强的抗菌活性,其相对分子质量约为14 k Da。下层条带主要色谱峰出现在5、35、43、45、46、47、50 min,其中35、43、45 min收集的样品有抗菌活性,其相对分子质量分别约为14-16、6和12 k Da。各个组分对大肠杆菌BL-21和金黄色葡萄球菌26003均具有抑菌活性。结论:从奶牛子宫内膜组织分离出了5个抗菌成分,从相对分子质量、抗菌活性等方面初步认定为抗菌多肽。
