生物化学代谢部分内容繁杂,包含大量的物质和代谢途径。仅通过一条条代谢路径的介绍很难使学生理解代谢途径的意义及相互联系。我们在生物化学授课过程中,以改变四大物质代谢适应其生长的肿瘤细胞为线,在糖、脂、氨基酸及核苷酸代谢中分别融入肿瘤细胞代谢的研究进展。当完成四大物质代谢学习后,学生课后以小组形式研读肿瘤代谢高引用文献综述"The emerging hallmarks of cancermetabolism",并根据文献设计问题,最后在课堂上以小组挑战赛的形式研讨文献。学生们通过研读文献及讨论,了解了肿瘤细胞调整代谢途径以适应其生长的策略,建立了物质代谢之间的相互联系,整合了代谢途径,充分认识到代谢途径的意义。
Chicken anterior pituitary extract(CAPE) and acetone dried chicken anterior pituitary (ACAPE) were injected intraperitoneally into normal laying hens (‘ovulation suppressed’ following pretreatment with daily subcutaneous injection of PMSG) to induce multiple ovulations. The dose of PMSG, the effect of CAPE and ACAPE and the time required for induction of ovulation following injection of ovulation inducing hormone were determined. The results revealed that (1) when 75 IU PMSG was administered daily, egg laying stopped in 33% of the treated hens within 6 days after the first injection. However, the percentage of hens showing the same effects changed significantly (over 95%) within 3 to 6 days when the amount of PMSG was increased to 100 IU; (2) the number of ovulated ova was 1 00±0 00, 2 33±0 26,2 20±0 20 respectively after receiving 100 mg, 200 mg and 300 mg; the number of ovulated ova was 2 00±0 00, 2 86±0 48, 3 00±1 50 respectively after receiving 10 mg, 15 mg and 20 mg ACAPE; (3) The time from injection to ovulation in almost all hens was about 7 5 h except one hen ovulated about 6 5 h after receiving ACAPE .
