A novel non-enzymatic method for the determination of oxidases and their sub-strates was proposed. The principle was examined by fluorometric determination of glucose oxidase(GOD). Using EGTA (NH4)2Fe (SO4), as modified Fenton reagent, H2O2, the prod-uct of GOD catalytic reaction was converted into hydroxyl radical, the later oxidized tereph-thalic acid, which was chosen from four fluorogenic indicator of hydroxyl radical, into fluo-rescent product GOD was thus measured according to the reaction rates. The rnethod is sim-ple, stable, cheap and at least as sensitive as its counterpart enzymatic method-
本文叙述了新建立的磁场效应(Magnetic Field Effects,简称MFE_s)-偏振-共振同步荧光法。利用偏振技术抑制掉溶剂散射光的99％,利用MFE_s抑制溶剂98％的散射光波动。将偏振、MFE_s技术与共振同步荧光法结合为一体时,则大大改善Δλ=0的共振同步荧光法的检测灵敏度。如蒽、苝舵同时测定时,其检测限分别为0.068ng/ml、0.015ng/ml。优于一般的Δλ值较小(0～5nm)的同步荧光法和偏振-同步荧光法。
