The solution structure of bovine cytochrome b 5 mutant (E44A/E48A/E56A/D60A) has been obtained from 1H NMR spectra measured at 600 MHz. The final RMSD values with respect to the average structure are (0.056±0.009) nm and (0.105±0.010) nm for backbone and all heavy atoms, respectively. The solution structure, when compared with the X ray structure of wild type, has no significant changes in the whole folding and secondary structure. Small changes are restricted to local conformation near mutated sites. [WT5HZ]
