Three new impurities of d-Biotin were synthesized:(2S,3S,4S)-5-(3,4-diamino-tetrahydro-thiophen-2-yl)-pentanoic acid,(3aS,4S,6aR)-hexahydro-1-thia-3,4a-diaza-cyclopenta inden-4-one and(3aS,4S,6aR)-4-(5’-Oxo-hexyl)-tetrahydro-thieno-imidazol-2-one.Structures of target compounds were confirmed by NMR and so on.Our work further improved the research on the impurities of d-biotin,and can be applied to the quality control and quality assurance of d-biotin.
Two impurities of d-biotin are synthesized:di[3-[(3aS,4S,6aR)-2-oxohexahydrothienoimidazol-4-yl]propyl]acetic acid,4-[(3aS,4S,6aR)-2-oxohexahydrothienoimidazol-4-yl]butane-1,1-dicarboxylic acid.Structure of target compounds are confirmed by 1HNMR.This work further improve the research on the impurities of d-biotin,and can be applied to the quality control and quality assurance of d-biotin.
