Two new species of the genus Eotmethis Bey-Bienko, 1948 are described in this paper, namely E. daqingshanensis sp. nov. and E. gansuensis sp. nov. from the Inner Mongolia Autonomous Region and Gansu Province, respectively. The type specimens of the new species are deposited in College of Life Sciences, Hebei University, Baoding, China.
Phylogenetic relationships of Pamphagidae were examined using cytochrome oxidase subunit I（cox1） gene sequences.Eleven species of Pamphagidae from 7 genera were sequenced to obtain mt DNA data,along with 2 species from the Gen Bank nucleotide database.The results of sequence comparisons showed the cox1 gene is 1 534 bp in length,including 326 varible sites and 211 parsimonious information sites.The percentage of A ＋T is 67.1% in the nucleotide sequences,showing a strong AT bias.Genetic distances among subfamilies are 0.08.Using Locusta migratoria as outgroup,the phylogenetic trees were reconstructed with NJ,MP,ML and Bayesian inferences,and the results showed that the clustering results were approximately identical to that of classical morphological classification.Thrinchinae and Pamphaginae were a monophyletic group,respectively.Two species of the genus Asiotmethis of Prionotropisinae did not get together with other species of Prionotropisinae,classification position of Asiotmethis should be further discussed by both genetic markers and morphological features.The current genus Filchnerella of Prionotropisinae was not a monophyletic group.
A new species, Humphaplotropis culaishanensis sp. nov. of the genus Humphaplotropis Xiao, ~in et Yin, 2013, is described from Shandong, China in this paper. The new species is similar to Humphaplotropis taishanensis Xiao, Yin et Yin, 2013, but differs from the latter by： both side carinae of frontal ridge distinct, widened gently downwards, not reaching the clypeus; pronotum hump along the median keel slightly, the over height above level line of vertex occupied 1/5 of whole high ofpronotum in lateral view; anterior margin acute angular in the middle, the apex not reaching the hind margin of eyes; tegmina longer, extending over the hind margin of first abdominal terga. Type specimens are deposited in the College of Plant Protection, Shandong Agricultural University, Taian, China.
记述了采自摩洛哥土尔蝗属Tuarega1新种,瓦尔扎扎特土尔蝗Tuarega ouarzazatensissp.nov.,模式标本保存于河北大学博物馆。尔瓦扎扎特土尔蝗,新种Tuarega ouarzazatensissp.nov.（图1-6）新种与Tuarega insignis（Lucas,1851）近似,区别特征为：前胸背板沟后区宽大于长,前翅中脉与肘脉不闭合,前翅肘脉有2个分支。正模♀,摩洛哥瓦尔扎扎特东南10km,海拔1100m,2009-03-04,Martin Husemann和Jan Habel博士（NaturalHistory Museum of Luxembourg）采。词源：新种种名出自摩洛哥的瓦尔扎扎特,模式标本产地。
Phylogenetic relationships of Pamphagidae were examined using cytochrome oxidase subunit II （COIl） mtDNA sequences （684 bp）. Twenty-seven species of Acridoidea from 20 genera were sequenced to obtain mtDNA data, along with four species from the GenBank nucleotide database. The purpose of this study was analyzing the phylogenetic relationships among subfamilies within Pamphagidae and interpreting the phylogenetic position of this family within the Acridoidea superfamily. Phylogenetic trees were reconstructed using neighbor-joining （NJ）, maximurn parsimony （MP） and Bayesian inference （BI） methods. The 684 bp analyzed fragment included 126 parsimony informative sites. Sequences diverged 1.0%-11.1% between genera within subfamilies, and 8.8%-12.3% between subfamilies. Amino acid sequence diverged 0-6.1% between genera within subfamilies, and 0.4%-7.5% between subfamilies. Our phylogenetic trees revealed the monophyly of Pamphagidae and three distinct major groups within this family. Moreover, several well supported and stable clades were found in Pamphagidae. The global clustering results were similar to that obtained through classical morphological classification： Prionotropisinae, Thrinchinae and Pamphaginae were monophyletic groups. However, the current genus Filchnerella （Prionotropisinae） was not a monophyletic group and the genus Asiotmethis （Prionotropisinae） was a sister group of the genus Thrinchus （Thrinchinae）. Further molec- ular and morphological studies are required to clarify the phylogenetic relationships of the genera Filchnerella and Asiotmethis.
