＜正＞Objective The present study was carried out to investigate the effect of electroacupuncture（EA）on paraventr...
Huan-Huan ZHANG~(1,2),Meng-Ya WANG~1,Da-Nian ZHU~(2,*) 1 Cell Electrophysiology Laboratory,Wannan Medical College,Wuhu 241002,China 2 Department of Physiology and Pathophysiology,Shanghai Medical College of Fudan University,Shanghai 200032, China
Acupuncture treatment is a great creation of ancient physicians in China, and acupuncture analgesia is the most influential therapy. After the founding of the People＇s Republic of China in 1949, the acupuncture analgesia of integrated traditional Chinese and Western medicine has obtained great development.
Objective:Cumulative evidences suggested that electroacupuncture(EA) could modulate immune function.Previous s...
Jing Li1,Sheng Xiao1,Hui Zhao1,Yan-Qing Wang1,2,Jun Wang1,*,Gen-Cheng Wu1,2,*(1 Department of Integrative Medicine and Neurobiology,Institute of Acupuncture Research WHO Collaborating Center for Traditional Medicine,Shanghai Medical School,Fudan University,Shanghai,200032,China 2 Institutes of Brain Science,State Key Laboratory of Medical Neurobiology,Fudan University,Shanghai,200032,China)
＜正＞Objective:Surgical trauma stress has been reported to induce immunosuppression.The mechanisms involved are ...
Jing LI~1,Sheng XIAO~1,Hui ZHAO~1,Yan-Qing WANG~(1,2),Jun WANG~(1,*),Gen-Cheng WU~(1,2,*) 1 Department of Integrative Medicine and Neurobiology,Institute of Acupuncture Research WHO Collaborating Center for Traditional Medicine,Shanghai Medical School.Fudan University,Shanghai 200032,China 2 Institutes of Brain Science,State Key Laboratory of Medical Neurobiology,Fudan University,Shanghai 200032, China
＜正＞Cumulative evidences suggest that electroacupuncture（EA） can modulate immune function,but the mechanism nee...
Jun WANG,Jing LI,Yan-Qing WANG,Gen-Cheng WU~* Department of Integrative Medicine and Neurobiology,Institute of Acupuncture Research WHO Collaborating Center for Traditional Medicine,Institutes of Brain Science,State Key Laboratory of Medical Neurobiology,Fudan University, Shanghai,200032,China
Objective To explore the role of the extracellular signal-regulated kinase （ERK）/cAMP response element binding protein （CREB） pathway in the induction of long-term potentiation （LTP） in the anterior cingulate cortex （ACC） that may be implicated in pain-related negative emotion. Methods LTP of field potential was recorded in ACC slice and the expressions of phospho-ERK （pERK） and phospho-CREB （pCREB） were examined using immunohistochemistry method. Results LTP could be induced stably in ACC slice by high frequency stimulation （2-train, 100 Hz, 1 s）, while APv （an antagonist of NMDA receptor） could block the induction of LTP in the ACC, indicating that LTP in this experiment was NMDA receptor-dependent. Bath application of PD98059 （50 μmol/L）, a selective MEK inhibitor, at 30 min before tetanic stimulation could completely block the induction of LTP. Moreover, the protein level of pERK in the ACC was transiently increased after LTP induction, starting at 5 rain and returning to basal at 1 h after tetanic stimulation. The protein level of pCREB was also increased after LTP induction. The up-regulation in pERK and pCREB expressions could be blocked by pretreatment of PD98059. Double immunostaining showed that after LTP induction, most pERK was co-localized with pCREB. Conclusion NMDA receptor and ERK-CREB pathway are necessary for the induction of LTP in rat ACC and may play important roles in pain emotion.
