Ancient DNA molecules were successfully extracted from remains of Laoshang t omb. PCR amplification and sequencing analysis of mitochondrial hypervariable re gion Ⅰ (16035-16398) were carried out to determine its haplogroup. The results indicate the sequence belongs to M haplogroup attributed to Asian which consist s with the study of physical anthropology. The project acertained its lineage an d meanwhile enriched the database of ancient populations in China.
