[Objective] The aim was to study the effect of the content of copper and zinc on in medium the vitamin E accumulation in wheat embryo-dreived callus.[Method] The mathematical models were established to describe the growth kinetics and the vitamin E accumulation in wheat embryo callus cells.With the aim of getting the highest accumulation of the secondary metabolite Vitamin E,the optimal combination of copper and zinc in medium was confirmed by testing.[Result] The results showed that the production of vitamin E in B5 medium reached the highest value with 2.0 mg/mL ZnSO4·7H2O and 0.1 mg/mL CuSO4·5H2O.The fitting degrees of kinetic models of vitamin E accumulation and cell growth were 97.53% and 95.60%,respectively,which indicated good nonlinear relationships.[Conclusion] Both copper and zinc could affect the accumulation of vitamin E in wheat germ callus,and Copper showed more prominent effect than Zn.Synergism existed in low copper and zinc concentration,and the inhibitive effect enhanced with the increase of the concentrations.
