A simple, sensitive and specific high performance liquid chromatography-tandem mass spectrometry （HPLC-MS/MS）method combined with solid phase extraction has been developed and validated for the simultaneous quantification of paeoniflorinand albiflorin in rats plasma and tissue homogenate after administration of total glycosides of paeony （TGP）. Chromatographicseparation was achieved on a C15 column （150 mmx4.6 mm, 5 pro） with mobile phase consisted of acetonitrile-0.05% formicacid aqueous （20： 80, v/v） at a flow rate of 0.5 mL/min. The analytes were detected with triple-quadrupole mass spectrometer usingturbo ion spray with negative ionization in the multiple reaction-monitoring （MRM） modes. The results of method validationconformed to the requirements for the determination of biological samples. This method was successfully applied to thepharmacokinetics and tissue distribution study of TGP in rats. The results showed that paeonifiorin and albiflorin wereabsorbed and reached peak value quickly, and had the similar pharmacokinetic characteristics. Both of them underwent a rapid andwide distribution in the tissues throughout the whole body, among which stomach was the main distribution tissue. Moreover, theyhad the ability to cross the blood-brain barrier.
目的建立液相色谱-串联质谱法同时测定地榆槐角丸中芦丁、槐角苷、柚皮苷、染料木苷、黄芩苷、染料木素、芦荟大黄素、大黄酸和大黄素含量的方法。方法采用C18色谱柱,乙腈-0.1%乙酸水为流动相,梯度洗脱。采用电喷雾离子源,监测模式为负离子,定量检测采用多重反应离子模式。结果在15min内地榆槐角丸中9种有效成分在所测定的浓度范围内线性关系均良好,加样回收率(n=3)分别为96.2%、97.5%、98.3%、99.6%、97.5%、100.1%、94.2%、98.7%和99.7%,相对标准偏差(relative standard deviation,RSD)值分别为1.3%、1.2%、1.4%、1.7%、0.5%、0.54%、0.9%、1.0%和0.82%。结论该方法简便、准确、重现性好、专属性高,可用于地榆槐角丸的质量控制。
